Journal: Oxidative Medicine and Cellular Longevity

Article Title: Qinbaohong Zhike Oral Liquid Attenuates LPS-Induced Acute Lung Injury in Immature Rats by Inhibiting OLFM4

doi: 10.1155/2022/7272371

Figure Lengend Snippet: Functional analysis of QBH-regulated DEPs in the lung tissue determined by quantitative proteomics. (a) Functional GO enrichment analysis of the DEPs according to the classification of BP, CC and MF. (b) Functional KEGG enrichment analysis showing the top 20 signaling pathways. (c) The PPI network analysis generated by two different comparisons. (d) Abundances of OLFM4 reported by TMT-based quantitative proteomic result. Data are presented as mean ± SD ( n = 5/group). ∗ p < 0.05 vs. control group; # p < 0.05 vs. ALI group.

Article Snippet: After blocking with 5% non-fat milk at 4°C overnight, the membrane was incubated with primary antibody olfactomedin 4 (cat. No. bs-6558R, 1 : 1,000, Bioss, Beijing, China) overnight at 4°C. β -actin (cat. No. 3700, 1 : 1,000, Cell Signaling Technology, MA, USA) was used as an internal control.

Techniques: Functional Assay, Generated

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Qinbaohong Zhike Oral Liquid Attenuates LPS-Induced Acute Lung Injury in Immature Rats by Inhibiting OLFM4

doi: 10.1155/2022/7272371

Figure Lengend Snippet: Validation of QBH on the expression of OLFM4 in the lung tissue. (a) Representative images of OLFM4 expression determined by western blot. (b) Relative protein expression level of OLFM4. Data are presented as mean ± SD ( n = 3/group). ∗∗ p < 0.01 vs. control group; # p < 0.05 and ## p < 0.01 vs. ALI group.

Article Snippet: After blocking with 5% non-fat milk at 4°C overnight, the membrane was incubated with primary antibody olfactomedin 4 (cat. No. bs-6558R, 1 : 1,000, Bioss, Beijing, China) overnight at 4°C. β -actin (cat. No. 3700, 1 : 1,000, Cell Signaling Technology, MA, USA) was used as an internal control.

Techniques: Expressing, Western Blot

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: Expression and cellular distribution of OLFM4 in tumor and nontumor tissue samples. ( a , b ). Staining of OLFM4 in HCC cells and in nontumor cells: proportion of samples showing no staining (none), cytoplasmic staining (cytoplasm only), membrane staining (membrane only), or both (m + c). Representative cytoplasm staining pattern in HCC samples: ( c ) score 0, negative; ( d ) score 1, weak; ( e ) score 2, strong. Representative typical positive membrane staining in HCC tissues: ( f ) score 0, negative; ( g ) score 1, less than 30% of cells; ( h ) 30%–70% of cells; ( i ) over 70% of cells. Magnification, ×40. HCC, hepatocellular carcinoma.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: Expressing, Staining, Membrane

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: Assessment of the correlations between OLFM4 and E-cadherin ( a ) or MMP-7 ( b ) mRNA expression levels according to the analysis of an independent HCC cohort. HCC, hepatocellular carcinoma.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: Expressing

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: Prognostic significance of OLFM4 according to cellular distribution in patients undergoing hepatectomy. ( a ) Overall survival (OS) of the entire patients' collective as determined by the Kaplan-Meier method. (b) OS according to evidence of vessel invasion. OS according to cytoplasm staining for OLFM4 (positive or negative ( c )) or according to semiquantitative assessment of staining intensity ( d ). OS according to membrane staining for OLFM4 (positive or negative ( e )) or according to semiquantitative assessment of staining intensity ( f ). ( g ) OS according to OLFM4 staining in HCC tissues from LMU Munich. ( h ) Kaplan-Meier survival curves according to OLFM4 mRNA expression levels in a second independent cohort of patients with HCC from TCGA. +Censored cases. HCC, hepatocellular carcinoma; TCGA, The Cancer Genome Atlas.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: Staining, Membrane, Expressing

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: Overall survival according to OLFM4 staining in non-HCC tissues from LMU Munich cohort of hepatectomy. +Censored cases. HCC, hepatocellular carcinoma.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: Staining

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: In vitro assessment of the effect of OLFM4 silencing on MMP-7 and E-cadherin. ( a ) Effect of transfection of Huh7 and PLCPRF5 cells with siRNA specifically targeting OLFM4 or non-coding siRNA (Beta-gal, 50 nM) for 24 hours on the expression of the indicated molecules after immunoblotting. ( b ) Typical pattern of OLFM4 expression at immunofluorescence after transfection with control-targeting or OLFM4-targeting siRNA. Green: DAPI staining of the nuclei; Red: immunofluorescence of OLFM4.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: In Vitro, Transfection, Expressing, Western Blot, Immunofluorescence, Control, Staining

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: Effect of OLFM4 silencing on cell migration and invasion. ( a , b ) Assessment of cell motility by wound healing assay in Huh7 or PLCPRF5 cells transfected with siRNA-OLFM4 or control-si-RNA. ( c ) Quantitative analysis of healing area in percentages. ( d , e ) The migration and invasion of Huh7 and PLCPRF5 cells transfected with siRNA-OLFM4 compared with the controls by transwell assay after 24 hours. ( f , g ) Quantitative analysis of migration and invasion cells. ( h ) The E-cadherin expression of Huh7 and PLCPRF5 cells transfected with siRNA-OLFM4 compared with the controls by immunofluorescence after 24 hours. ( i – j ) Effects of OLFM4 silencing by siRNA on sub-G1 events, cell cycle distribution, and cell viability in Huh7 ( i ) and PLCPRF5 ( j ) cell lines. Values represented mean ± SD from 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, Student t -test. Blue: DAPI staining of nuclei; green, immunofluorescence of E-cadherin.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: Migration, Wound Healing Assay, Transfection, Control, Transwell Assay, Expressing, Immunofluorescence, Staining

Journal: Clinical and Translational Gastroenterology

Article Title: Prognostic Significance and Functional Relevance of Olfactomedin 4 in Early-Stage Hepatocellular Carcinoma

doi: 10.14309/ctg.0000000000000124

Figure Lengend Snippet: Schematic diagram describing the hypothesized effect of OLFM4, MMP family members and on invasion and metastasis formation in HCC. HCC, hepatocellular carcinoma.

Article Snippet: Anti-OLFM4 polyclonal rabbit antibody (BIOZOL GmbH, Eching, Germany), anti-MMP-7 monoclonal mouse antibody (Merck KgaA, Gernsheim, Germany), and anti-E-cadherin monoclonal mouse antibody (Cell Signaling Technology, Danvers, MA) were applied as primary antibodies.

Techniques: